Franken Daniel R, Menkveld Roelof, Kruger Thinus F, Sekadde-Kigondu Christine, Lombard Carl
Reproductive Biology Research Laboratory, Department of Obstetrics and Gynaecology, University of Stellenbosch, Tygerberg, South Africa.
Fertil Steril. 2003 Jun;79 Suppl 3:1637-43. doi: 10.1016/s0015-0282(03)00367-4.
The value of sperm morphology as a predictor of a man's fertilizing potential has often been challenged because of different classification systems used to distinguish between normal and abnormal spermatozoa. The study aimed to monitor the reading skills of 53 laboratory technologists who are enrolled in a continuous quality control program for sperm morphology assessment.
Prospective analytical study.
Academic hospital and academic institution setting.
PATIENT(S): Sperm samples from the sperm donor program and andrology technicians from sub-Saharan Africa.
INTERVENTION(S): Papanicolaou-stained sperm slides were prepared and shipped on a quarterly basis to participating laboratories. Papanicolaou-prestained sperm morphology slides were used as test material for 21 months.
MAIN OUTCOME MEASURE(S): Percentage normal spermatozoa.A new statistical model was developed to record reading skills of the participating technicians. Reading skills were classified as marginal (5.7% of cases), good (11.3% of cases), and excellent (83% of cases).
RESULT(S): Participants maintained their morphological reading skills and agreed with the reference laboratory by not exceeding a SD limit of 0.2 to set stringent standards for the program.
CONCLUSION(S): Technician proficiency can be monitored using the results of a quality control program. A continuous quality control program can be initiated only after intensive training, because baseline values at the onset of the quality control program serves as an internal reference value.
由于用于区分正常和异常精子的分类系统不同，精子形态作为男性受精潜力预测指标的价值常受到质疑。本研究旨在监测53名参与精子形态评估持续质量控制项目的实验室技术人员的阅片技能。
前瞻性分析研究。
学术医院和学术机构环境。
来自精子捐赠项目的精子样本以及撒哈拉以南非洲的男科技术人员。
制备巴氏染色的精子玻片，并每季度将其运送到参与的实验室。巴氏预染色的精子形态玻片用作测试材料，为期21个月。
正常精子百分比。开发了一种新的统计模型来记录参与技术人员的阅片技能。阅片技能分为边缘水平（5.7%的病例）、良好水平（11.3%的病例）和优秀水平（83%的病例）。
参与者保持了他们的形态学阅片技能，并且与参考实验室一致，未超过0.2的标准差限值，从而为该项目设定了严格的标准。
可使用质量控制项目的结果来监测技术人员的熟练程度。只有在强化培训后才能启动持续质量控制项目，因为质量控制项目开始时的基线值可作为内部参考值。
