Neuwinger J, Behre H M, Nieschlag E
Institute of Reproductive Medicine, University of Münster, Federal Republic of Germany.
Fertil Steril. 1990 Aug;54(2):308-14.
An external quality control study for semen analysis was performed involving 10 andrology laboratories in geographically separate locations. For the evaluation of sperm concentration, eight samples with different concentrations, and for the assessment of sperm morphology three slides prepared from different semen samples were distributed. Sperm motility was evaluated in five samples delivered cryopreserved to the participants. The coefficients of variation (CVs) for sperm counts varied with the sperm concentrations showing highest variability for low and lowest for high concentrations (range 23% to 73%). The CV for sperm morphology ranged from 25% for normal heads to 87% for abnormal midpieces. The CV for motility of sperm was 21%. For comparison the mean CVs for internal quality control were as follows: 10% for concentration, 8% for morphology (normal heads), and 8% for motility.
开展了一项精液分析外部质量控制研究，涉及位于不同地理位置的10个男科实验室。为评估精子浓度，分发了8个不同浓度的样本；为评估精子形态，分发了由不同精液样本制备的3张载玻片。对5个冷冻保存后交付给参与者的样本进行了精子活力评估。精子计数的变异系数（CV）随精子浓度而变化，低浓度时变异性最高，高浓度时最低（范围为23%至73%）。精子形态的CV范围为正常头部25%至异常中段87%。精子活力的CV为21%。作为比较，内部质量控制的平均CV如下：浓度为10%，形态（正常头部）为8%，活力为8%。
