Grant A, McDonnell L
Centre for Surface & Interface Analysis, Department of Applied Physics and Instrumentation, Cork Institute of Technology, Bishopstown, Cork, Ireland.
Ultramicroscopy. 2003 Oct-Nov;97(1-4):177-84. doi: 10.1016/S0304-3991(03)00042-1.
Given that biological samples are particularly soft in their natural state and that a liquid is the most difficult operating environment for non-contact mode scanning force microscopy, the development of a scanning force microscope that is optimised to image biological samples in vitro presents significant challenges. The performance of the instrument described here has been optimised for such an application by incorporating magnetic excitation and active-Q control of the cantilever. The application of this instrument has been validated by imaging monolayers of immobilised antibodies in liquid and achieving image qualities comparable to those achieved in air for such samples.
鉴于生物样品在自然状态下特别柔软，且液体是用于非接触模式扫描力显微镜的最具挑战性的操作环境，开发一种优化用于体外对生物样品成像的扫描力显微镜面临重大挑战。此处描述的仪器通过结合悬臂的磁激励和有源Q控制，针对此类应用进行了性能优化。通过对液体中固定化抗体单层进行成像并获得与此类样品在空气中成像时相当的图像质量，验证了该仪器的应用效果。
