Jiao Yuekan, Schäffer Tilman E
Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology, Am Fassberg 11, 37077 Göttingen, Germany.
Langmuir. 2004 Nov 9;20(23):10038-45. doi: 10.1021/la048650u.
The suitability of three common atomic force microscope (AFM) imaging modes for quantitative height and volume measurements on soft samples was investigated. The height and volume of rehydrated human metaphase chromosomes in liquid were measured using the contact mode, the tapping mode, and the force mapping mode. In both the contact and tapping modes, the measured height and volume strongly depended on the imaging setpoint that sets the imaging force. Measurement deviations up to 50% were observed. The force mapping mode, on the other hand, yielded reproducible height and volume measurements independent of the imaging force. It is therefore suggested that the force mapping mode should be used whenever the height or volume of soft samples need to be accurately determined.
研究了三种常见的原子力显微镜（AFM）成像模式对软样品进行定量高度和体积测量的适用性。使用接触模式、轻敲模式和力映射模式测量了液体中复水的人类中期染色体的高度和体积。在接触模式和轻敲模式下，测量的高度和体积强烈依赖于设定成像力的成像设定点。观察到测量偏差高达50%。另一方面，力映射模式产生了与成像力无关的可重复的高度和体积测量结果。因此，建议在需要准确测定软样品的高度或体积时使用力映射模式。
