Finot Eric, Bourillot Eric, Meunier-Prest Rita, Lacroute Yvon, Legay Guillaume, Cherkaoui-Malki Mustapha, Latruffe Norbert, Siri Olivier, Braunstein Pierre, Dereux Alain
Laboratoire de Physique, Université de Bourgogne, UMR CNRS 5027, Université de Bourgogne, F-21011, Dijon, France.
Ultramicroscopy. 2003 Oct-Nov;97(1-4):441-9. doi: 10.1016/S0304-3991(03)00072-X.
An electrochemical methodology for bio-molecule sensing using an array of well-defined nanostructures is presented. We describe the fabrication by e-beam lithography of nanoelectrodes consisting of a 100 micro m x 50 micro m area containing interdigitated electrodes of 100 nm in width and interelectrode distance of 200 nm. Sensitivity and response time of the nanoelectrodes are compared to the responses of macro- and microelectrodes. The specificity of the sensor is studied by modifying the gold electrodes with DNA. The technique enables to characterize both single and double-stranded DNA of 15 nucleotides. A special electrochemical cell is adapted to control the temperature and measure the DNA concentration by UV analysis. The electrochemical method requires no label on the DNA, only redox mediators were used.
本文介绍了一种使用一系列定义明确的纳米结构进行生物分子传感的电化学方法。我们描述了通过电子束光刻制造纳米电极的过程，该纳米电极的面积为100μm×50μm，包含宽度为100nm且电极间距为200nm的叉指电极。将纳米电极的灵敏度和响应时间与宏观电极和微电极的响应进行了比较。通过用DNA修饰金电极来研究传感器的特异性。该技术能够对15个核苷酸的单链和双链DNA进行表征。一种特殊的电化学池适用于控制温度并通过紫外分析测量DNA浓度。该电化学方法不需要对DNA进行标记，仅使用了氧化还原介质。
