Khudoerkov R M
Biull Eksp Biol Med. 1992 Jun;113(6):660-3.
An alkaline range pH 9.1-12.3 instead of values pH 7.0-7.1 was used to localize lysine-rich and arginine-rich nucleoproteins by ammonia silver (AS) staining method (Black and Ansley, 1966) in brain structures of rabbits and rats. This modification of AS method made it steady to reveal lysine-rich and arginine-rich nucleoproteins and was able to demonstrate the high variability of protein localization in relation to the balance of essential amino acids in the brain structures with different functions. The best results of morphological staining of nerve, glial cells and their fibres were found at pH 10.6--the point of maximal dissociation of lysine--NH2 groups. At the same time the new modification of AS method allows the use of all alkaline range pH 9.1-12.3 for revealing the cells of nervous tissue and to vary the treatment of sections with silver ions.
通过氨银（AS）染色法（Black和Ansley，1966），在兔和大鼠的脑结构中，使用pH 9.1 - 12.3的碱性范围而非pH 7.0 - 7.1的值来定位富含赖氨酸和精氨酸的核蛋白。AS方法的这种改进使得揭示富含赖氨酸和精氨酸的核蛋白变得稳定，并且能够证明与具有不同功能的脑结构中必需氨基酸平衡相关的蛋白质定位的高度变异性。在pH 10.6（赖氨酸 - NH2基团的最大解离点）时，发现神经、胶质细胞及其纤维的形态染色效果最佳。同时，AS方法的新改进允许在pH 9.1 - 12.3的整个碱性范围内用于显示神经组织细胞，并可改变切片与银离子的处理方式。
