Staykova M, Jordanov J, Goranov I
Z Mikrosk Anat Forsch. 1978;92(3):571-6.
A modification of Black and Ansley's ammoniacal silver reaction (ASR) for histones is proposed for visualizing myelin basic protien (MBP) in the nervous system. The reaction is performed on histological sections of tissues fixed in neutralized formalin-alcohol and delipidized in the course of the routine paraffin embedding. The deparaffinized sections are again treated with formalin in order to make the "unmasked" by the delipidization basic groups of MBP reactive to ammoniacal silver. After treatment with this reagent MBP of the myelin sheaths of the nerve fibres is impregnated brownish-black. Deparaffinized sections subjected to an extraction of MBP with hydrochloric acid exhibit a negative reaction at the level of the myelin sheaths the same reaction being preserved at the level of the nuclear histones. The reaction is positive in paper spots of nervous tissue extracts obtained with the same acid. These assays indicate the specificity of the modified ASR. The method can be used for studies on the processes of myelination and demylination in normal histogenesis and in pathology of the nervous tissue.
提出了一种对布莱克和安斯利用于组蛋白的氨性银反应（ASR）的改良方法，用于在神经系统中可视化髓鞘碱性蛋白（MBP）。该反应在经中和福尔马林 - 酒精固定并在常规石蜡包埋过程中脱脂的组织学切片上进行。脱石蜡切片再次用福尔马林处理，以使经脱脂后“暴露”的MBP碱性基团对氨性银具有反应性。用该试剂处理后，神经纤维髓鞘的MBP被染成棕黑色。用盐酸提取MBP的脱石蜡切片在髓鞘水平呈现阴性反应，而在核组蛋白水平保持相同反应。用相同酸获得的神经组织提取物的纸斑反应呈阳性。这些试验表明改良的ASR具有特异性。该方法可用于研究正常组织发生和神经组织病理学中髓鞘形成和脱髓鞘过程。
