Corbin I R, Buist R, Peeling J, Zhang M, Uhanova J, Minuk G Y
Section of Hepatology, Department of Medicine, University of Manitoba, Winnipeg, Canada.
Gut. 2003 Jul;52(7):1046-53. doi: 10.1136/gut.52.7.1046.
Hepatic adenosine triphosphate (ATP) levels are an accurate reflection of functioning hepatic mass following surgical resections and acute liver injury.
To determine whether hepatic ATP levels can serve as a non-invasive means of documenting progression of chronic liver disease to cirrhosis.
In vivo phosphorus-31 magnetic resonance spectroscopy ((31)P MRS) was performed in three animal models of chronic liver disease. Sixty six adult Sprague- Dawley rats were subjected to either thioacetamide, carbon tetrachloride (CCl(4)), or common bile duct ligation (CBDL) to induce liver disease (n=35, 21, and 10, respectively). Serial MRS examinations, blood samples, and liver biopsies (when appropriate) were obtained throughout and/or on completion of the study.
Over the course of the chronic liver disease, a progressive decrease in hepatic ATP levels was consistently observed in each model. The findings were most striking when end stage liver disease (cirrhosis) was established. The reduction in hepatic ATP levels correlated with significant changes in serum albumin concentrations (CCl(4) and CBDL models) and the extent of hepatocyte loss seen histologically (all models).
The results of this study indicate that during progression of chronic liver disease to cirrhosis, there is a progressive reduction in hepatic ATP levels. In addition, changes in hepatic ATP levels correlate with changes in liver function and histology. Thus hepatic (31)P MRS provides a non-invasive means of documenting the severity and progression of parenchymal and cholestatic models of chronic liver disease in rats.
肝三磷酸腺苷（ATP）水平是手术切除和急性肝损伤后肝实质功能的准确反映。
确定肝ATP水平是否可作为记录慢性肝病进展为肝硬化的一种非侵入性方法。
在三种慢性肝病动物模型中进行体内磷-31磁共振波谱（（31）P MRS）检查。66只成年Sprague-Dawley大鼠分别接受硫代乙酰胺、四氯化碳（CCl4）或胆总管结扎术（CBDL）以诱导肝病（分别为n = 35、21和10）。在研究过程中和/或结束时获取系列MRS检查、血样和肝活检标本（如适用）。
在慢性肝病过程中，每个模型均持续观察到肝ATP水平逐渐下降。在终末期肝病（肝硬化）形成时，这一发现最为显著。肝ATP水平的降低与血清白蛋白浓度的显著变化（CCl4和CBDL模型）以及组织学上观察到的肝细胞丢失程度（所有模型）相关。
本研究结果表明，在慢性肝病进展为肝硬化的过程中，肝ATP水平逐渐降低。此外，肝ATP水平的变化与肝功能和组织学变化相关。因此，肝（31）P MRS提供了一种非侵入性方法来记录大鼠慢性肝病实质和胆汁淤积模型的严重程度和进展情况。
