Yomogida Kentaro, Yagura Yo, Tadokoro Yuko, Nishimune Yoshitake
Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
Biol Reprod. 2003 Oct;69(4):1303-7. doi: 10.1095/biolreprod.103.015958. Epub 2003 Jun 11.
Although the mammalian germinal stem cell (GSC) provides a good model to investigate the regulation of stem cells, the small number of these cells currently available hampers elucidation of the regulatory mechanism. Here, we show the dramatic amplification of GSCs in mouse testis following transfection of human glial cell line-derived neurotrophic factor cDNA into Sertoli cells using an efficient, in vivo electroporation technique. Transplantation analysis demonstrated not only GSC enrichment but also differentiation from stem cells into sperm. The GSC population, as estimated using a colony-formation assay, was approximately 20-fold greater than in cryptorchid testis, or approximately 500- to 1000-fold greater than in normal adult testis. This system should provide sufficient quantities of GSCs to accelerate our understanding of GSC properties, regulation mechanisms, and behavior control.
尽管哺乳动物生殖干细胞（GSC）为研究干细胞的调控提供了一个良好的模型，但目前可获得的这类细胞数量较少，这妨碍了对调控机制的阐明。在此，我们展示了使用高效的体内电穿孔技术将人胶质细胞系衍生的神经营养因子cDNA转染到支持细胞后，小鼠睾丸中GSC的显著扩增。移植分析不仅证明了GSC的富集，还证明了干细胞向精子的分化。使用集落形成试验估计，GSC群体比隐睾中的大约大20倍，比正常成年睾丸中的大约大500至1000倍。该系统应能提供足够数量的GSC，以加速我们对GSC特性、调控机制和行为控制的理解。
