Asai J, Fukushima Y, Makino Y
Department of Neurosurgery, Fujigaoka Hospital, Showa University, Yokohama.
Neurol Med Chir (Tokyo). 1992 Oct;32(11):811-7. doi: 10.2176/nmc.32.811.
Proteins from 21 cerebrospinal fluid (CSF) samples (14 derived from neurological cases and 7 from normal individuals) and 15 serum samples (11 from neurological cases and 4 from normal individuals) were analyzed by two-dimensional electrophoresis. Protein mapping revealed a very acidic protein (Ac-P) at about pH 3.5 in the 71% of CSF samples from neurological cases. However, no serum sample contained Ac-P. Ac-P was isolated and purified, and determined to be a glycoprotein containing a large amount of carbohydrate, with molecular weight 42,000 and isoelectric point 2.7-3.3. The amino acid composition of Ac-P was consistent with alpha 1-acid glycoprotein (AGP), and Ac-P was responsive to a commercial anti-AGP antiserum in the radial immunodiffusion test. The known polymorphism of AGP suggests some differences in physicochemical properties such as molecular weight and isoelectric point between AGP in serum and in CSF. Quantitative analysis of Ac-P (AGP) and total protein levels in CSF showed a partial interdependence. Ac-P may be a useful marker for detecting a pathological conditions of the central nervous system.
采用双向电泳对来自21份脑脊液（CSF）样本（14份来自神经病例，7份来自正常个体）和15份血清样本（11份来自神经病例，4份来自正常个体）中的蛋白质进行分析。蛋白质图谱显示，在71%的神经病例CSF样本中，约pH 3.5处有一个非常酸性的蛋白质（Ac-P）。然而，血清样本中均未含有Ac-P。对Ac-P进行了分离和纯化，确定其为一种含有大量碳水化合物的糖蛋白，分子量为42,000，等电点为2.7 - 3.3。Ac-P的氨基酸组成与α1-酸性糖蛋白（AGP）一致，且在放射免疫扩散试验中，Ac-P对市售抗AGP抗血清有反应。已知AGP的多态性表明，血清和CSF中的AGP在诸如分子量和等电点等理化性质上存在一些差异。CSF中Ac-P（AGP）和总蛋白水平的定量分析显示存在部分相互依存关系。Ac-P可能是检测中枢神经系统病理状况的有用标志物。
