犬α-1-酸性糖蛋白的分离、表征及定量分析

Isolation, characterization and quantitation of canine alpha-1-acid glycoprotein.

作者信息

Yuki M, Itoh H, Tamura K, Nishii N, Takase K

机构信息

Yuki Animal Hospital, 2-99 Kiba-cho, Minato-ku, Nagoya, Aichi, 455-0021, Japan.

出版信息

Vet Res Commun. 2008 Oct;32(7):533-42. doi: 10.1007/s11259-008-9055-9. Epub 2008 Jul 16.

DOI:10.1007/s11259-008-9055-9

PMID:18629604

Abstract

The present study was designed to investigate the physicochemical properties of canine alpha-1-acid glycoprotein (AGP), and to establish a method for measuring serum AGP in canine. Fifty-three normal beagles were used in the study. AGP was purified from normal canine sera by successive ammonium sulfate precipitation, ion-exchange chromatography and gel filtration. The serum AGP concentration was measured by single radial immunodiffusion (SRID). Canine AGP had a molecular weight of 42,000 +/- 2,000 Da and contained 40.9% carbohydrate. Gel isoelectric focusing revealed microheterogeneity with 6-7 bands in a pI range (isoelectric points) of 3.2-3.8. AGP migrated to the alpha1-globulin region on immunoelectrophoresis. The serum AGP level of 35 normal beagles was 283.4 +/- 113.3 microg/ml. Canine AGP was isolated, and its physicochemical properties were clarified. SRID may be a useful method for quantification of serum AGP in canine.

摘要

本研究旨在调查犬α-1-酸性糖蛋白（AGP）的理化性质，并建立一种测定犬血清AGP的方法。本研究使用了53只正常的比格犬。通过连续硫酸铵沉淀、离子交换色谱和凝胶过滤从正常犬血清中纯化AGP。血清AGP浓度通过单向放射免疫扩散法（SRID）测定。犬AGP的分子量为42,000±2,000 Da，含40.9%的碳水化合物。凝胶等电聚焦显示在3.2 - 3.8的pI范围（等电点）内有6 - 7条带的微异质性。AGP在免疫电泳中迁移至α1球蛋白区域。35只正常比格犬的血清AGP水平为283.4±113.3μg/ml。犬AGP被分离出来，其理化性质得以阐明。SRID可能是一种用于定量犬血清AGP的有用方法。