Kobayashi M, Takamatsu K, Saitoh S, Miura M, Noguchi T
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Biochem Biophys Res Commun. 1992 Nov 30;189(1):511-7. doi: 10.1016/0006-291x(92)91587-g.
We have isolated a cDNA clone encoding a novel calcium-binding protein of the recoverin family from rat brain cDNA library. This clone (PCB11) has 588 nucleotides in the open reading frame including the termination codon, 174 nucleotides of the 5' leader and 800 nucleotides of the 3' noncoding region. The complete amino acid sequence deduced from the cDNA is composed of 195 residues, has a calculated molecular mass of 22,574 Daltons, and contains three putative calcium-binding domains of the EF-hand structure. The deduced amino acid sequence has a striking sequence homology to those of the retinal recoverin family (recoverin, visinin, P26, 23kD protein, S-modulin) and the brain-derived recoverin family (P23k, 21-kDa CaBP and neurocalcin). Northern blot, in situ hybridization, immunoblot and immunohistochemical analyses revealed that the protein is exclusively expressed in pyramidal layer of the hippocampus. The protein was therefore designated hippocalcin.
我们从大鼠脑cDNA文库中分离出一个编码恢复蛋白家族新型钙结合蛋白的cDNA克隆。该克隆（PCB11）在开放阅读框中含有588个核苷酸，包括终止密码子，5'前导序列有174个核苷酸，3'非编码区有800个核苷酸。从cDNA推导的完整氨基酸序列由195个残基组成，计算分子量为22,574道尔顿，并包含三个假定的EF手结构钙结合结构域。推导的氨基酸序列与视网膜恢复蛋白家族（恢复蛋白、视锥蛋白、P26、23kD蛋白、S-调钙蛋白）和脑源性恢复蛋白家族（P23k、21-kDa钙结合蛋白和神经钙蛋白）的序列具有显著的同源性。Northern印迹、原位杂交、免疫印迹和免疫组织化学分析表明，该蛋白仅在海马锥体层表达。因此，该蛋白被命名为海马钙结合蛋白。
