Takamatsu K, Noguchi T
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Neurosci Res. 1993 Sep;17(4):291-5. doi: 10.1016/0168-0102(93)90112-4.
Hippocalcin is a recently identified Ca(2+)-binding protein with three EF-hand structures, dominantly expressed in the hippocampal pyramidal layer. The complete amino acid sequence of hippocalcin deduced from the cDNA is composed of 195 residues, has a calculated molecular mass of 22,574 daltons, and has a striking sequence homology to those of visinin, recoverin, S-modulin, neurocalcins and neural visinin-like proteins. Hippocalcin binds 3 mol of Ca2+ per mol of protein at submicromolar Ca2+ levels, and associates the plasma membrane in a Ca(2+)-dependent manner. Hippocalcin is myristoylated at its NH2-terminal glycine residue, and this modification is a key event in terms of its membrane-association property.
海马钙结合蛋白是一种最近发现的具有三个EF手结构的钙结合蛋白，主要在海马锥体细胞层表达。从cDNA推导的海马钙结合蛋白的完整氨基酸序列由195个残基组成，计算分子量为22,574道尔顿，与视锥蛋白、恢复蛋白、S-调钙蛋白、神经钙蛋白和神经视锥蛋白样蛋白具有显著的序列同源性。在亚微摩尔钙水平下，海马钙结合蛋白每摩尔蛋白质结合3摩尔钙离子，并以钙依赖的方式与质膜结合。海马钙结合蛋白在其氨基末端甘氨酸残基处被豆蔻酰化，这种修饰是其膜结合特性的关键事件。
