小鼠精子发生相关环状指基因znf230的分子克隆与特性分析
Molecular cloning and characterization of a mouse spermatogenesis-related ring finger gene znf230.
作者信息
Qiu Weimin, Zhang Sizhong, Xiao Cuiying, Xu Wenmin, Ma Yongxin, Liu Yunqiang, Wu Qiaqing
机构信息
Department of Medical Genetics, West China Hospital, Sichuan University, Renminnanlu No. 17, Section 3, 610041, Chengdu, PR China.
出版信息
Biochem Biophys Res Commun. 2003 Jun 27;306(2):347-53. doi: 10.1016/s0006-291x(03)00970-7.
Complete cDNA of mouse gene znf230 was cloned by rapid amplification of cDNA ends (RACE). This cDNA is 982 base pairs (bp) in length and encodes a 230 amino acids (aa) protein with a ring finger domain at its C-terminus. Ninety-one and 98% nucleotide (nt) and aa sequence identity are observed with its human homolog. Revealed by Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR), this cDNA is only detected in testicular tissue, whereas the longer transcripts of 2.4 and 4.4kb are ubiquitously expressed. The expression of znf230 in testis is developmentally regulated and first detected at day 6 postnatal (pn). It reaches adult level between day 14 and 21 pn during which round spermatids appear in seminiferous tubule. The protein of znf230 exhibits DNA binding activity and its ring finger domain may function as an activator module in transcription. Therefore, it is postulated that znf230 may function as a testis specific transcription factor during mouse spermatogenesis.
通过cDNA末端快速扩增（RACE）技术克隆了小鼠基因znf230的完整cDNA。该cDNA长度为982个碱基对（bp），编码一个230个氨基酸（aa）的蛋白质，其C末端具有一个锌指结构域。与人类同源物相比，核苷酸（nt）和氨基酸序列的同一性分别为91%和98%。通过Northern印迹和逆转录聚合酶链反应（RT-PCR）发现，该cDNA仅在睾丸组织中检测到，而2.4kb和4.4kb的较长转录本在全身广泛表达。znf230在睾丸中的表达受发育调控，在出生后第6天（pn）首次检测到。在第14至21天pn期间达到成年水平，在此期间曲细精管中出现圆形精子细胞。znf230蛋白具有DNA结合活性，其锌指结构域可能在转录中作为激活模块发挥作用。因此，推测znf230在小鼠精子发生过程中可能作为睾丸特异性转录因子发挥作用。
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