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分子克隆与表达分析：斑马鱼新型锌指蛋白基因 rnf141

Molecular cloning and expression analysis of a zebrafish novel zinc finger protein gene rnf141.

机构信息

Department of Medical Genetics, West China Hospital Division of Morbid Genomics, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan People's Republic Of China.

出版信息

Genet Mol Biol. 2009 Jul;32(3):594-600. doi: 10.1590/S1415-47572009005000062. Epub 2009 Sep 1.

DOI:10.1590/S1415-47572009005000062
PMID:21637524
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3036043/
Abstract

ZNF230 is a novel zinc finger gene cloned by our laboratory. In order to understand the potential functions of this gene in vertebrate development, we cloned the zebrafish orthologue of human ZNF230, named rnf141. The cDNA fragment of rnf141 was obtained by rapid amplification of cDNA ends (RACE). The open reading frame (ORF) encodes a polypeptide of 222 amino acids which shares 75.65% identity with the human ZNF230. RT-PCR analysis in zebrafish embryo and adult tissues revealed that rnf141 transcripts are maternally derived and that rnf141 mRNA has a broad distribution. Zygotic rnf141 message is strongly localized in the central nervous system, as shown by whole-mount in situ hybridization. Knockdown and over expression of rnf141 can induce abnormal phenotypes, including abnormal development of brain, as well as yolk sac and axis extendsion. Marker gene analysis showed that rnf141 may play a role in normal dorsoventral patterning of zebrafish embryos, suggesting that rnf141 may have a broad function during early development of vertebrates.

摘要

ZNF230 是我们实验室克隆的一种新型锌指基因。为了了解该基因在脊椎动物发育中的潜在功能，我们克隆了人类 ZNF230 的斑马鱼同源基因，命名为 rnf141。通过快速扩增 cDNA 末端 (RACE) 获得了 rnf141 的 cDNA 片段。ORF 编码一个 222 个氨基酸的多肽，与人类 ZNF230 具有 75.65%的同一性。在斑马鱼胚胎和成年组织中的 RT-PCR 分析表明，rnf141 转录物是母源衍生的，rnf141 mRNA 具有广泛的分布。如整体原位杂交所示，合子 rnf141 消息强烈定位于中枢神经系统。rnf141 的敲低和过表达可诱导异常表型，包括脑、卵黄囊和轴的异常发育。标记基因分析表明，rnf141 可能在斑马鱼胚胎的正常背腹模式形成中发挥作用，这表明 rnf141 在脊椎动物早期发育中可能具有广泛的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/06033e8849de/gmb-32-3-594-gfig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/626676c58eca/gmb-32-3-594-gfig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/6df16e8f833a/gmb-32-3-594-gfig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/87f99a1778ef/gmb-32-3-594-gfig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/0a4c8e1468fe/gmb-32-3-594-gfig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/06033e8849de/gmb-32-3-594-gfig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/626676c58eca/gmb-32-3-594-gfig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/6df16e8f833a/gmb-32-3-594-gfig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/87f99a1778ef/gmb-32-3-594-gfig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/0a4c8e1468fe/gmb-32-3-594-gfig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b881/3036043/06033e8849de/gmb-32-3-594-gfig5.jpg

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本文引用的文献

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Zebrafish spata2 is expressed at early developmental stages.斑马鱼spata2在发育早期阶段表达。
Int J Dev Biol. 2007;51(3):241-6. doi: 10.1387/ijdb.062220em.
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