Douay L
Service d'hématologie biologique, Hôpital d'enfants Armand Trousseau, 75012 Paris.
Ann Biol Clin (Paris). 2003 May-Jun;61(3):259-67.
The ability to identify, select and manipulate stem cells from tissues opens the way to regenerative medicine. In the field of hematopoiesis, our increasingly precise knowledge of the mechanisms of production and regulation of blood cells should permit in the near future the production of cells in sufficient quantity to be of interest for transfusion. Our present capacity to amplify hematopoietic stem cells and direct their differentiation towards the erythroid line is reviewed here. A procedure is described for the amplification of CD34 + hematopoietic stem cells of placental origin, based on the sequential use of specific combinations of growth factors in a given serum-free medium. Such conditions allowing the terminal maturation in vivo of large numbers of precursors produced ex vivo permit one to envisage important transfusion perspectives. A complementary source of red blood cells would in fact improve our transfusion capacity.
从组织中识别、选择和操控干细胞的能力为再生医学开辟了道路。在造血领域，我们对血细胞生成和调控机制的了解日益精确，这有望在不久的将来生产出足够数量的细胞用于输血。本文综述了我们目前扩增造血干细胞并使其向红系分化的能力。描述了一种基于在特定无血清培养基中依次使用生长因子特定组合来扩增胎盘来源的CD34 +造血干细胞的方法。这种条件允许大量体外产生的前体细胞在体内终末成熟，从而使人能够设想重要的输血前景。事实上，红细胞的补充来源将提高我们的输血能力。
