Yang Shi, Cai Haibo, Jin Huili, Tan Wen-Song
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Biotechnol Lett. 2008 Jan;30(1):61-5. doi: 10.1007/s10529-007-9517-0. Epub 2007 Sep 11.
The hematopoietic reconstitution of cord blood (CB) CD34(+)cells grown in static and stirred system was studied. Static cultures were better than stirred cultures for cell expansion. Engraftment of stirred-culture hematopoietic stem cells (HSCs) was higher than static-culture HSCs. Stirred-culture HSCs had better multilineage reconstitution ability and colony-forming ability than static-culture HSCs. Static cultures thus favor the expansion of HSCs and stirred cultures are more effective in preserving functional HSCs.
研究了在静态和搅拌系统中培养的脐带血（CB）CD34(+)细胞的造血重建情况。在细胞扩增方面，静态培养优于搅拌培养。搅拌培养的造血干细胞（HSCs）的植入率高于静态培养的HSCs。与静态培养的HSCs相比，搅拌培养的HSCs具有更好的多系重建能力和集落形成能力。因此，静态培养有利于HSCs的扩增，而搅拌培养在保存功能性HSCs方面更有效。
