通过制备型SDS-PAGE对软体动物贝壳基质进行表征
Molluscan shell matrix characterization by preparative SDS-PAGE.
作者信息
Marin Frédéric
机构信息
IsoTis, NV., ProfBronkhorstlaan, 10, Gebouw D, 3723 MB Bilthoven, The Netherlands.
出版信息
ScientificWorldJournal. 2003 May 5;3:342-7. doi: 10.1100/tsw.2003.30.
The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated "blind" on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.
软体动物贝壳基质中的糖蛋白成分通常难以通过色谱法进行分离。我们描述了一种方法，该方法克服了这一困难，并能够收集大量的贝壳蛋白用于进一步的体外特性分析。在矿物相溶解后，糖蛋白在制备型电泳上进行“盲法”分离。随后用针对整个基质产生的多克隆抗体对它们进行检测。
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