Hasegawa Masakazu, Kagiyama Soichiro, Tajima Masaru, Yoshida Kazuya, Minami Yayoi, Kurosawa Tsutomu
Technical Research Laboratory, Dai-Dan Co., Ltd., 390 Kitanagai, Miyoshi-machi, Iruma-gun, Saitama 354-00440, Japan.
Exp Anim. 2003 Apr;52(2):145-51. doi: 10.1538/expanim.52.145.
Studies to date have established that the physical environment inside cages can be controlled adequately by setting the intra-cage ventilation at 60 air changes per hour in a forced-air-ventilated micro-isolation system (FVMIS). In this study, the capability of FVMIS to prevent inter-cage transmission of microorganisms was evaluated using Pasteurella pneumotropica as a reference microorganism. One FVMIS rack and a conventional rack were used, and cages with mice positive for P. pneumotropica and those with P. pneumotropica-free mice were housed on both racks. The mice were examined for P. pneumotropica contamination every 4 weeks after initiating the experiment for 12 weeks using a polymerase chain reaction method. Some P. pneumotropica-free mice housed in open air cages in the conventional rack became positive for P. pneumotropica (four of 28 animals after 4 weeks; eight of 28 animals after 12 weeks), but all P. pneumotropica-free mice housed in the FVMIS cages remained negative for the bacterium throughout the experiment. The results demonstrate that FVMIS can prevent inter-cage transmission of P. pneumotropica when proper cage handling practice is under taken.
迄今为止的研究表明，在强制通风的微隔离系统（FVMIS）中，通过将笼内通风设置为每小时60次换气，可以充分控制笼内的物理环境。在本研究中，以嗜肺巴斯德氏菌作为参考微生物，评估了FVMIS防止微生物在笼间传播的能力。使用了一个FVMIS架和一个传统架，在这两个架子上都饲养了感染嗜肺巴斯德氏菌呈阳性的小鼠的笼子和饲养无嗜肺巴斯德氏菌小鼠的笼子。在使用聚合酶链反应方法进行为期12周的实验后，每4周对小鼠进行一次嗜肺巴斯德氏菌污染检测。在传统架上开放式笼子中饲养的一些无嗜肺巴斯德氏菌小鼠感染了嗜肺巴斯德氏菌（4周后28只动物中有4只；12周后28只动物中有8只），但在整个实验过程中，饲养在FVMIS笼子中的所有无嗜肺巴斯德氏菌小鼠对该细菌检测仍呈阴性。结果表明，当采取适当的笼具处理措施时，FVMIS可以防止嗜肺巴斯德氏菌在笼间传播。
