Chang Wen-Hsiang, Chang Yen, Lai Po-Hong, Sung Hsing-Wen
Department of Chemical Engineering. National Tsing Hua University, Hsinchu, Taiwan 30013, ROC.
J Biomater Sci Polym Ed. 2003;14(5):481-95. doi: 10.1163/156856203766652084.
A naturally occurring crosslinking agent (genipin) was used in this study to crosslink gelatin hydrogel to develop a wound-dressing membrane. The study was to investigate the in vitro characteristics of the genipin-crosslinked gelatin membrane. The glutaraldehyde-crosslinked counterpart, at a similar crosslinking degree, was used as control. Additionally, an in vivo experiment was undertaken to study the wound healings covered with the glutaraldehyde- and genipin-crosslinked dressings in a rat model. The in vitro results obtained suggested that crosslinking of gelatin membranes with glutaraldehyde or genipin may produce distinct crosslinking structures. The differences in crosslinking structure can significantly affect the mechanical property, water-vapor-transmission rate, swelling ratio, degradation against enzyme and cellular compatibility of the crosslinked membranes. In the in vivo study, it was found that the degree of inflammatory reaction for the wound treated with the genipin-crosslinked dressing was significantly less severe than that covered with the glutaraldehyde-crosslinked dressing throughout the entire course of the study. Additionally, the healing rate for the wound treated with the genipin-crosslinked dressing was notably faster than its glutaraldehyde-crosslinked counterpart.
本研究使用一种天然存在的交联剂（京尼平）对明胶水凝胶进行交联，以制备伤口敷料膜。该研究旨在探究京尼平交联明胶膜的体外特性。将具有相似交联度的戊二醛交联对照物用作对照。此外，还进行了一项体内实验，以研究大鼠模型中用戊二醛和京尼平交联敷料覆盖的伤口愈合情况。获得的体外结果表明，用戊二醛或京尼平交联明胶膜可能会产生不同的交联结构。交联结构的差异会显著影响交联膜的机械性能、水汽透过率、溶胀率、酶降解性和细胞相容性。在体内研究中发现，在整个研究过程中，用京尼平交联敷料处理的伤口的炎症反应程度明显低于用戊二醛交联敷料覆盖的伤口。此外，用京尼平交联敷料处理的伤口的愈合速度明显快于其戊二醛交联对照物。
