Yeolekar L R, Damle R G, Basu A, Rao B L
National Institute of Virology (ICMR), Pune, India.
Indian J Med Res. 2002 Dec;116:255-7.
Mink lung epithelial cells (Mv-1-Lu) were tested for their ability to support the growth and serial passage of respiratory syncytial virus (RSV) in vitro. Indian isolates of RSV induced distinctive cytopathic effect with typical rounding of cells followed by detachment with more than 50 per cent cells showing bright fluorescence using anti-RSV monoclonal antibodies in immunofluorescence test. Serial passage of RSV was possible in Mv-1-Lu cells without loss of sensitivity of the cells for virus growth. Titration of cell associated virus and virus released in the supernatant indicated that 60 per cent of the virus was released in the supernatant, and 40 per cent remained cell associated. Transmission electron microscopic studies of negatively stained RSV particles and ultra-thin sections of RSV infected Mv-1-Lu cells showed roughly spherical particles with club shaped projections, budding from the cytoplasmic membrane. These results indicate that Mv-1-Lu cell line is suitable for the growth and propagation of RSV.
对貂肺上皮细胞（Mv-1-Lu）支持呼吸道合胞病毒（RSV）体外生长和连续传代的能力进行了检测。印度分离的RSV诱导出独特的细胞病变效应，细胞典型地变圆，随后脱落，在免疫荧光试验中，超过50%的细胞使用抗RSV单克隆抗体显示明亮荧光。RSV能够在Mv-1-Lu细胞中连续传代，且细胞对病毒生长的敏感性不丧失。对细胞相关病毒和上清液中释放的病毒进行滴定表明，60%的病毒释放到上清液中，40%仍与细胞相关。对负染的RSV颗粒以及RSV感染的Mv-1-Lu细胞超薄切片进行的透射电子显微镜研究显示，病毒颗粒大致呈球形，带有棒状突起，从细胞膜出芽。这些结果表明，Mv-1-Lu细胞系适合RSV的生长和繁殖。
