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一种与哺乳动物SRP54同源的支原体蛋白识别SRP RNA的一个高度保守结构域。

A Mycoplasma protein homologous to mammalian SRP54 recognizes a highly conserved domain of SRP RNA.

作者信息

Samuelsson T

机构信息

Department of Medical Biochemistry, University of Göteborg, Sweden.

出版信息

Nucleic Acids Res. 1992 Nov 11;20(21):5763-70. doi: 10.1093/nar/20.21.5763.

DOI:10.1093/nar/20.21.5763
PMID:1280809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334414/
Abstract

A protein homologous to SRP54, a subunit of the mammalian signal recognition particle (SRP), was identified in Mycoplasma mycoides. The mycoplasma protein was expressed in E.coli and purified to near homogeneity. It was shown to bind specifically in vitro to a small mycoplasma RNA with structural features related to the RNA component of SRP. These findings provide evidence of a ribonucleoprotein complex in mycoplasma reminiscent of SRP. A part of the RNA was protected from ribonuclease digestion in the presence of the SRP54 homologue. The protected region contains structural elements that have been highly conserved in SRP RNAs during evolution.

摘要

在蕈状支原体中鉴定出一种与哺乳动物信号识别颗粒（SRP）的亚基SRP54同源的蛋白质。该支原体蛋白在大肠杆菌中表达并纯化至近乎同质。结果表明，它在体外能特异性结合一种小型支原体RNA，该RNA具有与SRP的RNA组分相关的结构特征。这些发现为支原体中存在一种类似于SRP的核糖核蛋白复合体提供了证据。在SRP54同源物存在的情况下，部分RNA可免受核糖核酸酶消化。受保护区域包含在进化过程中SRP RNA中高度保守的结构元件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d72/334414/91f260cf640e/nar00232-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d72/334414/9294779cf29a/nar00232-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d72/334414/91f260cf640e/nar00232-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d72/334414/9294779cf29a/nar00232-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d72/334414/91f260cf640e/nar00232-0248-b.jpg

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