蕈状支原体转运RNA基因的克隆与核苷酸序列分析
Cloning and nucleotide sequence analysis of transfer RNA genes from Mycoplasma mycoides.
作者信息
Samuelsson T, Elias P, Lustig F, Guindy Y S
出版信息
Biochem J. 1985 Nov 15;232(1):223-8. doi: 10.1042/bj2320223.
Abstract
As part of an investigation of the tRNA genes of Mycoplasma mycoides, two HindIII fragments of mycoplasma DNA comprising 0.4 and 2.5 kilobases (kb), respectively, were cloned in pBR322 and their nucleotide sequences determined. Only one tRNA gene was found in the 0.4 kb fragment, the gene for tRNAArg with the anticodon TCT, while the 2.5 kb fragment contained nine different tRNA genes arranged in a cluster which presumably constitutes a transcriptional unit. The clustered tRNA genes, with their respective anticodons, were as follows: Arg (ACG), Pro (TGG), Ala (TGC), Met (CAT), Ile (CAT), Ser (TGA), fMet (CAT), Asp (GTC), and Phe (GAA).
摘要
作为对蕈状支原体tRNA基因研究的一部分，分别包含0.4和2.5千碱基（kb）的两个支原体DNA的HindIII片段被克隆到pBR322中，并测定了它们的核苷酸序列。在0.4 kb片段中仅发现一个tRNA基因，即反密码子为TCT的tRNAArg基因，而2.5 kb片段包含九个不同的tRNA基因，它们成簇排列，推测构成一个转录单元。这些成簇的tRNA基因及其各自的反密码子如下：Arg（ACG）、Pro（TGG）、Ala（TGC）、Met（CAT）、Ile（CAT）、Ser（TGA）、fMet（CAT）、Asp（GTC）和Phe（GAA）。
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