人前列腺癌腔内晶体的免疫组织化学和超微结构研究。

Immunohistochemical and ultrastructural studies of intraluminal crystalloids in human prostatic carcinomas.

作者信息

Ohtsuki Y, Furihata M, Inoue K, Iwata J, Manabe Y, Sonobe H, Ochi K, Seike H, Hashimoto H, Terao N

机构信息

Department of Pathology, Kochi Medical School, Japan.

出版信息

Virchows Arch A Pathol Anat Histopathol. 1992;421(5):421-5. doi: 10.1007/BF01606915.

DOI:10.1007/BF01606915

PMID:1280882

Abstract

Intraluminal crystalloids (ICr) observed in 19 cases of incidental or invasive human prostatic carcinoma (PCa) and in a case of benign prostatic hyperplasia were examined extensively by immunohistochemistry and electron microscopy. They were brilliantly eosinophilic with haematoxylin and eosin, manifesting needle-like, triangular, rectangular, hexagonal and irregular lump-like in shape. They were strongly positive, dark blue, with phosphotungstic acid -haematoxylin (PTAH) stain in all cases examined. Among the human antibodies tested, epithelial membrane antigen (EMA) gave specifically positive immunostainability with ICr in all cases. Annual ring-like lamellar or concentric structures were detected by electron microscopy. Positive staining of ICr with PTAH and anti-EMA antibody is very useful as a diagnostic marker for PCa in human prostatic tissues.

摘要

对19例偶然发现或侵袭性前列腺癌（PCa）以及1例良性前列腺增生病例中观察到的管腔内晶体（ICr）进行了免疫组织化学和电子显微镜的广泛检查。它们在苏木精和伊红染色下呈明亮的嗜酸性，形状为针状、三角形、矩形、六边形和不规则块状。在所有检查病例中，它们用磷钨酸苏木精（PTAH）染色均呈强阳性、深蓝色。在所测试的人类抗体中，上皮膜抗原（EMA）在所有病例中对ICr均呈现特异性阳性免疫染色性。通过电子显微镜检测到年轮状层状或同心结构。ICr用PTAH和抗EMA抗体的阳性染色作为人类前列腺组织中PCa的诊断标志物非常有用。