Schweppe Rebecca E, Haydon Claire E, Lewis Timothy S, Resing Katheryn A, Ahn Natalie G
Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA.
Acc Chem Res. 2003 Jun;36(6):453-61. doi: 10.1021/ar020143l.
Most biological processes are regulated by post-translational modifications of proteins, and conditions that disrupt the regulation of such events can lead to disease. In the past decade, the identification and characterization of covalent modifications have been driven by advances in mass spectrometry. Here, we discuss current mass spectrometric and proteomic approaches for the identification of proteins and their covalent modifications, and we highlight high-throughput strategies for comprehensive analysis of cell proteomes.
大多数生物过程受蛋白质翻译后修饰的调控，而扰乱此类事件调控的状况可能会导致疾病。在过去十年中，共价修饰的鉴定与表征工作因质谱技术的进步而得以推进。在此，我们讨论用于鉴定蛋白质及其共价修饰的当前质谱和蛋白质组学方法，并着重介绍用于细胞蛋白质组全面分析的高通量策略。
