Favrin Stacy J, Jassim Sabah A, Griffiths Mansel W
Department of Food Science, University of Guelph, Guelph, ON, Canada N1G 2W1.
Int J Food Microbiol. 2003 Aug 15;85(1-2):63-71. doi: 10.1016/s0168-1605(02)00483-x.
Salmonella infection is the second most prevalent cause of foodborne illness in most developing countries. Meat, poultry, and dairy products are frequently implicated in outbreaks. The objective of this study was to apply a novel immunomagnetic separation (IMS)-bacteriophage assay to the detection of Salmonella enteritidis in artificially inoculated skimmed milk powder, chicken rinses, and ground beef. In all food types tested, the IMS-bacteriophage assay was able to detect an average of 3 CFU of S. enteritidis in 25 g or ml of food sample. Total assay time including pre-enrichment is about 20 h. The results indicate that the IMS-bacteriophage assay is a rapid and sensitive means of detecting S. enteritidis in these foods. The assay was successfully adapted to the detection of Escherichia coli O157:H7 and was able to detect E. coli in ground beef at the lowest inoculation level tested, 2 CFU/g. The assay was also adapted to the simultaneous detection of S. enteritidis and E. coli. The results indicate that the IMS-bacteriophage assay shows promise for the simultaneous detection of these pathogens, but further development work would be necessary to improve sensitivity and produce reliable results at low inoculation levels.
在大多数发展中国家，沙门氏菌感染是食源性疾病的第二大常见病因。肉类、禽类和乳制品经常与疫情爆发有关。本研究的目的是将一种新型免疫磁珠分离（IMS）-噬菌体检测法应用于人工接种的脱脂奶粉、鸡肉冲洗液和绞碎牛肉中肠炎沙门氏菌的检测。在所有测试的食品类型中，IMS-噬菌体检测法能够在25克或25毫升食品样品中平均检测到3个肠炎沙门氏菌菌落形成单位（CFU）。包括预富集在内的总检测时间约为20小时。结果表明，IMS-噬菌体检测法是检测这些食品中肠炎沙门氏菌的一种快速且灵敏的方法。该检测法已成功应用于检测大肠杆菌O157:H7，并且能够在测试的最低接种水平（2 CFU/克）下检测到绞碎牛肉中的大肠杆菌。该检测法还适用于同时检测肠炎沙门氏菌和大肠杆菌。结果表明，IMS-噬菌体检测法在同时检测这些病原体方面显示出前景，但需要进一步开展研发工作以提高灵敏度，并在低接种水平下产生可靠结果。
