Schmelcher Mathias, Loessner Martin J
Institute of Food, Nutrition and Health; ETH Zurich; Zurich, Switzerland.
Bacteriophage. 2014 Jan 1;4(1):e28137. doi: 10.4161/bact.28137. Epub 2014 Feb 7.
Bacterial contamination of food products presents a challenge for the food industry and poses a high risk for the consumer. Despite increasing awareness and improved hygiene measures, foodborne pathogens remain a threat for public health, and novel methods for detection of these organisms are needed. Bacteriophages represent ideal tools for diagnostic assays because of their high target cell specificity, inherent signal-amplifying properties, easy and inexpensive production, and robustness. Every stage of the phage lytic multiplication cycle, from the initial recognition of the host cell to the final lysis event, may be harnessed in several ways for the purpose of bacterial detection. Besides intact phage particles, phage-derived affinity molecules such as cell wall binding domains and receptor binding proteins can serve for this purpose. This review provides an overview of existing phage-based technologies for detection of foodborne pathogens, and highlights the most recent developments in this field, with particular emphasis on phage-based biosensors.
食品产品的细菌污染给食品行业带来了挑战，并对消费者构成了高风险。尽管人们的认识不断提高，卫生措施也有所改善，但食源性病原体仍然对公众健康构成威胁，因此需要新的方法来检测这些微生物。噬菌体因其高靶细胞特异性、固有的信号放大特性、易于生产且成本低廉以及稳定性，是诊断检测的理想工具。从宿主细胞的初始识别到最终裂解事件，噬菌体裂解增殖周期的每个阶段都可以通过多种方式用于细菌检测。除了完整的噬菌体颗粒外，噬菌体衍生的亲和分子如细胞壁结合域和受体结合蛋白也可用于此目的。本文综述了现有的基于噬菌体的食源性病原体检测技术，并重点介绍了该领域的最新进展，特别强调了基于噬菌体的生物传感器。
