Zeiss C R, Hatoum N S, Ferguson J, Trout J, Levitz D, Siddiqui F, Henderson J, Yermakoff J, Patterson R
Department of Veterans Affairs Lakeside Medical Center, Chicago, IL 60611.
J Allergy Clin Immunol. 1992 Dec;90(6 Pt 1):944-52. doi: 10.1016/0091-6749(92)90467-g.
Male Sprague-Dawley rats were exposed to trimellitic anhydride (TMA) by inhalation (500 micrograms/m3), 4 hours a day, for 1 to 10 days. TMA was localized to lung cells by immunoelectron microscopy. Enzyme-linked immunosorbent assay (ELISA) and Western blot analysis were used in an attempt to localize TMA to lung lavage proteins. The lung-associated lymph node (LALN) B-lymphocyte response was measured by quantitation of immunoglobulin (Ig)G, IgA, and IgM antibody secreting cells specific for TMA rat serum albumin (TM-RSA) by use of the enzyme-linked immunospot assay (ELISPOT) method. The IgG, IgM, and IgA antibody response to TM-RSA in serum and lavage fluid was quantitated by ELISA. Lung injury was assessed by the number of external lung hemorrhagic foci and lung weight. Immunoelectron microscopy localized TMA to alveolar and bronchial cells on all exposure days. ELISA detected trace amounts of TMA haptenized lavage proteins that could not be detected by Western blot analysis. A marked increase occurred in lung injury from day 7 to 10. The LALN IgG, IgA, and IgM antibody secreting cell response to TM-RSA paralleled measures of lung injury. IgG, IgM, and IgA serum and lavage antibody to TM-RSA were correlated with lung injury measures. Lavage and serum IgG antibody levels had the highest correlation with lung injury.
将雄性Sprague-Dawley大鼠暴露于偏苯三酸酐（TMA）中，通过吸入（500微克/立方米），每天4小时，持续1至10天。通过免疫电子显微镜将TMA定位到肺细胞。使用酶联免疫吸附测定（ELISA）和蛋白质印迹分析试图将TMA定位到肺灌洗蛋白。通过使用酶联免疫斑点测定（ELISPOT）方法，通过定量针对TMA大鼠血清白蛋白（TM-RSA）的免疫球蛋白（Ig）G、IgA和IgM抗体分泌细胞来测量肺相关淋巴结（LALN）B淋巴细胞反应。通过ELISA定量血清和灌洗液中对TM-RSA的IgG、IgM和IgA抗体反应。通过外部肺出血灶数量和肺重量评估肺损伤。在所有暴露日，免疫电子显微镜将TMA定位到肺泡和支气管细胞。ELISA检测到痕量的与灌洗蛋白结合的TMA，而蛋白质印迹分析无法检测到这些结合物。从第7天到第10天，肺损伤显著增加。LALN对TM-RSA的IgG、IgA和IgM抗体分泌细胞反应与肺损伤指标平行。血清和灌洗液中针对TM-RSA的IgG、IgM和IgA抗体与肺损伤指标相关。灌洗液和血清IgG抗体水平与肺损伤的相关性最高。
