Pien L C, Zeiss C R, Leach C L, Hatoum N S, Levitz D, Garvin P J, Patterson R
Department of Medicine, Northwestern University Medical School, Chicago, Ill.
J Allergy Clin Immunol. 1988 Dec;82(6):1098-103. doi: 10.1016/0091-6749(88)90149-2.
Sprague-Dawley rats were exposed to trimellitic anhydride by inhalation, and the antibody response to trimellityl (TM)-conjugated hemoglobin (HB) and TM rat serum albumin (RSA) was compared. Groups of rats were exposed to trimellitic anhydride by inhalation 6 hours per day for 2, 6, or 10 days at 100 micrograms/m3 and compared to a control group exposed to filtered air. The IgG antibody response to TM-HB in both serum and bronchoalveolar lavage (BAL) fluid was measured with ELISA. IgG antibody levels to TM-HB rose significantly throughout the exposure. A positive correlation was found between IgG to TM-HB in serum and BAL fluid. In addition, this response in both serum and BAL fluid correlated with the IgG antibody response to TM-RSA. Cross-inhibition studies indicated the existence of shared antigenic determinants on TM-RSA and TM-HB. The IgG antibody to both antigens was specific for new antigenic determinants and not for the TM hapten.
将Sprague-Dawley大鼠通过吸入方式暴露于偏苯三酸酐中，并比较其对偏苯三酰（TM）偶联血红蛋白（HB）和TM大鼠血清白蛋白（RSA）的抗体反应。将大鼠分组，每天吸入偏苯三酸酐6小时，持续2、6或10天，浓度为100微克/立方米，并与暴露于过滤空气的对照组进行比较。用酶联免疫吸附测定法（ELISA）测量血清和支气管肺泡灌洗（BAL）液中对TM-HB的IgG抗体反应。在整个暴露过程中，血清中对TM-HB的IgG抗体反应显著升高。血清和BAL液中对TM-HB的IgG之间发现正相关。此外，血清和BAL液中的这种反应与对TM-RSA的IgG抗体反应相关。交叉抑制研究表明TM-RSA和TM-HB上存在共同的抗原决定簇。针对这两种抗原的IgG抗体对新的抗原决定簇具有特异性，而不是针对TM半抗原。
