Vrtacnik-Bokal Eda, Meden-Vrtovec Helena, Osredkar Josko, Verdenik Ivan
Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Slovenia.
Clin Chem Lab Med. 2003 May;41(5):663-7. doi: 10.1515/CCLM.2003.100.
The initial hypothesis was that the follicular fluid prorenin concentration was higher in women with polycystic ovaries (PCO) compared to normally cycling women stimulated by gonadotrophins. In a controlled prospective study, 47 women undergoing in vitro fertilisation (IVF) were enrolled: 20 women with PCO represented the study group and 27 normally cycling women the control group. Plasma prorenin, active renin, serum oestradiol, and androstenedione concentrations were measured on the day of human chorionic gonadotrophin (HCG) administration and 36 hours after HCG administration. Follicular fluid prorenin, active renin, oestradiol, and androstenedione concentrations from the dominant follicle and pooled follicles were measured 36 hours after HCG administration. Plasma prorenin concentration 36 hours after HCG administration was significantly higher in the PCO group (1867 microU/l; range 1137-3162 microU/l) than in controls (860 microU/l; range 433-1763 microU/l) (p = 0.007). The follicular fluid prorenin concentration in the dominant follicle and in the pooled follicles was lower in the PCO group (20190 microU/l; range 11130-25955 microU/l) than in controls (46930 microU/l; range 20671-66171 microU/l) (p = 0.003). We conclude that plasma prorenin concentrations in gonadotrophin-stimulated PCO cycles are augmented due to numerous follicles. Lower follicular fluid prorenin concentrations in women with PCO show that the synthesis of prorenin in theca cells starts later after HCG administration than in normally cycling women.
最初的假设是，与接受促性腺激素刺激的正常月经周期女性相比，多囊卵巢（PCO）女性的卵泡液中血管紧张素原浓度更高。在一项对照前瞻性研究中，招募了47名接受体外受精（IVF）的女性：20名PCO女性为研究组，27名正常月经周期女性为对照组。在注射人绒毛膜促性腺激素（HCG）当天以及注射HCG 36小时后，测量血浆血管紧张素原、活性肾素、血清雌二醇和雄烯二酮浓度。在注射HCG 36小时后，测量优势卵泡和集合卵泡中的卵泡液血管紧张素原、活性肾素、雌二醇和雄烯二酮浓度。PCO组在注射HCG 36小时后的血浆血管紧张素原浓度（1867微单位/升；范围1137 - 3162微单位/升）显著高于对照组（860微单位/升；范围433 - 1763微单位/升）（p = 0.007）。PCO组优势卵泡和集合卵泡中的卵泡液血管紧张素原浓度（20190微单位/升；范围11130 - 25955微单位/升）低于对照组（46930微单位/升；范围20671 - 66171微单位/升）（p = 0.003）。我们得出结论，由于卵泡数量众多，促性腺激素刺激下的PCO周期中血浆血管紧张素原浓度升高。PCO女性较低的卵泡液血管紧张素原浓度表明，与正常月经周期女性相比，PCO女性的卵泡膜细胞中血管紧张素原的合成在注射HCG后开始得更晚。
