Stand-off electrode (SoE): a new method for improving the sensitivity distribution of a tetrapolar probe.

Tetrapolar probes have been widely used for measuring the impedance spectra of tissues. However, the non-uniform sensitivity distribution of these probes limits the ability to identify conductivity changes in tissue. This paper presents a novel method for improving the sensitivity distribution beneath a tetrapolar probe. The method consists of placing a hydrogel layer between the probe and the tissue in order to make the sensitivity positive everywhere within the tissue. Theoretical and measured sensitivity distributions are compared. A good agreement between theoretical and measured data from an electrolytic tank was obtained with a maximum error of 1.3%. In vivo forearm measurements showed that the use of a conductive layer does enable tissue conductivity spectra to be determined. A smaller variation between subjects was obtained when using the stand-off. It was not possible to assess the absolute accuracy of the method due to the absence of a 'gold standard' for the measurement of tissue conductivity spectra.