梗阻性无精子症男性附睾精子的高性染色体非整倍体和二倍体率。
High sex chromosome aneuploidy and diploidy rate of epididymal spermatozoa in obstructive azoospermic men.
作者信息
Sukcharoen Nares, Ngeamvijawat Jiraporn, Sithipravej Tippawan, Promviengchai Sakchai
机构信息
Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
J Assist Reprod Genet. 2003 May;20(5):196-203. doi: 10.1023/a:1023674110940.
PURPOSE
To evaluate the frequencies of sex chromosome aneuploidy and diploidy rate of epididymal spermatozoa from obstructive azoospermic men and its impact on intracytoplasmic sperm injection (ICSI) outcomes.
METHODS
Epididymal spermatozoa retrieved from 24 obstructive azoospermic men and ejaculated spermatozoa from 24 fertile donors were analyzed using triple color fluorescence in situ hybridization (FISH) techniques, in order to investigate the rates of diploidy and aneuploidy for chromosomes 18, X and Y.
RESULTS
Epididymal spermatozoa from obstructive azoospermic men had total sex aneuploidy, disomy 18, and diploidy rates significantly higher than ejaculated spermatozoa from normozoospermic fertile controls (1.44% vs 0.14%, 0.11% vs 0.02%, and 0.18% vs 0.02%, respectively; p < 0.005). There were no statistically significant differences in ICSI outcomes between the patients who had high and low epididymal sperm aneuploidy rate.
CONCLUSIONS
Epididymal spermatozoa from obstructive azoospermic patients had an elevated sex chromosome aneuploidy and diploidy rate. The increased frequency of chromosomal abnormalities did not have a direct effect on the ICSI outcome.
目的
评估梗阻性无精子症男性附睾精子的性染色体非整倍体频率和二倍体率及其对卵胞浆内单精子注射（ICSI）结局的影响。
方法
采用三色荧光原位杂交（FISH）技术分析从24例梗阻性无精子症男性获取的附睾精子以及24例正常生育供者的射出精子，以研究18号、X和Y染色体的二倍体率和非整倍体率。
结果
梗阻性无精子症男性的附睾精子的总性染色体非整倍体、18号染色体双体和二倍体率显著高于正常精子的生育对照者的射出精子（分别为1.44%对0.14%、0.11%对0.02%、0.18%对0.02%；p<0.005）。附睾精子非整倍体率高和低的患者之间的ICSI结局无统计学显著差异。
结论
梗阻性无精子症患者的附睾精子的性染色体非整倍体和二倍体率升高。染色体异常频率增加对ICSI结局没有直接影响。
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