Bhattacharya M, Banerjee N, Ghosh D K
Department of Organic Chemistry (Carbohydrate), Indian Institute of Chemical Biology, Calcutta.
Ann Trop Med Parasitol. 1992 Aug;86(4):341-5. doi: 10.1080/00034983.1992.11812676.
A glycoconjugate antigen of 27-39 kDa was isolated from a cell-free extract of Leishmania donovani by affinity chromatography using a Concanavalin-A sepharose-4B column and eluted with 0.5 M alpha-methylmannoside. The antigen was recognized specifically by sera from kala-azar (visceral leishmaniasis) patients and did not react with sera from tuberculosis, leprosy or malaria patients. The antigen may therefore be useful in developing a serodiagnostic assay for visceral leishmaniasis.
通过使用伴刀豆球蛋白A琼脂糖-4B柱进行亲和层析，从杜氏利什曼原虫的无细胞提取物中分离出一种27 - 39 kDa的糖缀合物抗原，并用0.5 Mα-甲基甘露糖苷洗脱。该抗原能被黑热病（内脏利什曼病）患者的血清特异性识别，而不与结核病、麻风病或疟疾患者的血清发生反应。因此，该抗原可能有助于开发一种用于内脏利什曼病的血清诊断检测方法。
