Sinha R, Arora S K, Datta U, Sehgal S
Department of Immunopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Microbiol Immunol. 1992;36(4):391-400. doi: 10.1111/j.1348-0421.1992.tb02038.x.
Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42-116 kDa were selected as 'capture antibodies' and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients' sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was however low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.
制备了21种针对杜氏利什曼原虫前鞭毛体抗原的单克隆抗体。选择了5种单克隆抗体（Hyb.17、6、5、4和2），它们可识别与杜氏利什曼原虫42 - 116 kDa范围内各种抗原决定簇相关的分子，作为“捕获抗体”，并在竞争酶联免疫吸附测定系统（ELISA）中与特异性抗利什曼原虫抗血清比较，用于检测黑热病患者血清中的循环利什曼原虫抗原。抗利什曼原虫抗血清可在30%的黑热病病例中检测到循环抗原，而在这5种单克隆抗体中，Hyb.17能有效检测到85.4%的循环利什曼原虫抗原。然而，Hyb.6的检测效率较低（31.7%）。这些单克隆抗体在蛋白质印迹分析中识别的抗原可能代表黑热病患者血清中循环的抗原。在检测循环抗原用于黑热病临床诊断方面，这些单克隆抗体的混合物可能比传统的多克隆抗血清更有用。
