Shorrosh B S, Dixon R A
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73402.
Plant Mol Biol. 1992 Dec;20(5):801-7. doi: 10.1007/BF00027151.
A putative isocitrate dehydrogenase (IDH) cDNA from alfalfa has been cloned and sequenced. The derived amino acid sequence of 433 residues contains the isocitrate and isopropylmalate dehydrogenase signatures, is 63% identical to yeast mitochondrial NADP-IDH and shares high sequence identity with peptides of pig heart NADP-IDH. The sequence contains a potential N-terminal leader with similarities to a thylakoid transit peptide. IDH transcripts and NADP-IDH activity were detected in all alfalfa tissues examined, their levels depending upon the tissue type and its developmental stage. Transcripts and enzymatic activity were not induced on exposure of cell suspension cultures to a fungal elicitor. IDH is encoded by a small gene family in alfalfa.
已克隆并测序了来自苜蓿的一个假定异柠檬酸脱氢酶（IDH）cDNA。推导的433个氨基酸残基的序列包含异柠檬酸和异丙基苹果酸脱氢酶特征序列，与酵母线粒体NADP-IDH的同一性为63%，并与猪心NADP-IDH的肽段具有高度序列同一性。该序列含有一个潜在的N端前导序列，与类囊体转运肽相似。在所检测的所有苜蓿组织中均检测到IDH转录本和NADP-IDH活性，其水平取决于组织类型及其发育阶段。细胞悬浮培养物暴露于真菌激发子后，转录本和酶活性未被诱导。IDH在苜蓿中由一个小基因家族编码。
