Department of Plant Biology, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, England.
Plant Physiol. 1977 Apr;59(4):738-40. doi: 10.1104/pp.59.4.738.
Isocitrate dehydrogenase was found in Pisum sativum chloroplasts purified on sucrose density gradients. A chloroplast-enriched pellet obtained by differential centrifugation formed two chlorophyll-containing bands. The lower one containing intact chloroplasts had NADP-specific isocitrate dehydrogenase and triose-phosphate isomerase activities. Mitochondria and peroxisomes were observed to band well away from the intact chloroplast region, as indicated by peak activities of fumarase and catalase, respectively. The presence of isocitrate dehydrogenase in chloroplasts suggests that chloroplasts may generate at least some of the alpha-ketoglutarate required for glutamate synthesis.
在蔗糖密度梯度上纯化的豌豆叶绿体中发现了异柠檬酸脱氢酶。差速离心得到的富含叶绿体的沉淀形成了两条含叶绿素的条带。下面一条含有完整的叶绿体，具有 NADP 特异性异柠檬酸脱氢酶和磷酸丙糖异构酶活性。通过分别测定延胡索酸酶和过氧化氢酶的峰值活性，可以观察到线粒体和过氧化物体很好地远离完整叶绿体区域。叶绿体中存在异柠檬酸脱氢酶表明，叶绿体可能产生至少部分用于谷氨酸合成所需的α-酮戊二酸。
