Kennedy H E, Welsh M D, Cassidy J P, Bryson D G, Forster F, McNair J, Gangadharan B, Howard C J, Pollock J M
Department of Veterinary Science, Queen's University, Belfast, UK.
Vet Immunol Immunopathol. 2003 Jun 20;93(3-4):169-76. doi: 10.1016/s0165-2427(03)00070-9.
Delayed-type hypersensitivity (DTH) skin-testing with mycobacterial antigens is often used as a means of identifying Mycobacterium bovis-infected cattle. Better understanding of the cellular basis underlying the DTH reaction is required if diagnostic methods are to be improved upon. Previous studies have shown that gamma delta T-cells, particularly those bearing the WC1 molecule, are present at an early stage of developing DTH responses and that such cells may modulate the developing immune response immediately following M. bovis-infection. However, their role, if any, in the DTH response remains unclear. In the present study we have used an in vivo model to deplete WC1(+) gamma delta T-cells, from cattle with established M. bovis-infection, prior to skin-testing. Results indicate that, although WC1(+) gamma delta T-cells do infiltrate the skin-test site in normal calves, they do not appear to be essential for the development of DTH skin swelling, as indicated by effective development of skin responses in calves depleted of circulating WC1(+) gamma delta T-cells.
用分枝杆菌抗原进行迟发型超敏反应（DTH）皮肤试验常被用作鉴定牛分枝杆菌感染牛的一种方法。如果要改进诊断方法，就需要更好地理解DTH反应背后的细胞基础。先前的研究表明，γδ T细胞，尤其是那些携带WC1分子的细胞，在DTH反应发展的早期阶段就已存在，并且这些细胞可能在牛分枝杆菌感染后立即调节正在发展的免疫反应。然而，它们在DTH反应中的作用（如果有）仍不清楚。在本研究中，我们使用体内模型在皮肤试验前从已感染牛分枝杆菌的牛中清除WC1(+)γδ T细胞。结果表明，尽管WC1(+)γδ T细胞确实会浸润正常犊牛的皮肤试验部位，但如在清除循环WC1(+)γδ T细胞的犊牛中皮肤反应有效发展所示，它们似乎对DTH皮肤肿胀的发展并非必不可少。
