Suzuki Kiyoshi, Suzuki Yasuhiko, Kitamura Shinichi
Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuen-cho 1-1, Sakai, Osaka 599-8531, Japan.
J Exp Bot. 2003 Aug;54(389):1997-9. doi: 10.1093/jxb/erg203. Epub 2003 Jun 18.
A cDNA fragment was cloned from rice immature seeds by the RT-PCR method. The deduced amino acid sequence of the cDNA showed a high degree of identity with UDP-d-glucuronic acid decarboxylase (UXS) from other plants and was most similar to the soluble UXS from Arabidopsis. The recombinant protein, expressed in an Escherichia coli system, catalysed the conversion of UDP-d-glucuronic acid to UDP-d-xylose, confirming that the gene encoded UXS. The uxs gene was expressed in mature, harvested rice seeds as well as in immature seeds 14 d post-anthesis, suggesting that the uxs gene is necessary at the beginning of the germination period. This is the first report of the cloning of the uxs gene from monocots.
通过RT-PCR方法从水稻未成熟种子中克隆到一个cDNA片段。该cDNA推导的氨基酸序列与其他植物的UDP-D-葡萄糖醛酸脱羧酶（UXS）具有高度同源性，且与拟南芥的可溶性UXS最为相似。在大肠杆菌系统中表达的重组蛋白催化UDP-D-葡萄糖醛酸转化为UDP-D-木糖，证实该基因编码UXS。uxs基因在成熟收获的水稻种子以及花后14天的未成熟种子中均有表达，表明uxs基因在萌发期开始时是必需的。这是单子叶植物中uxs基因克隆的首次报道。
