Goto H, Shimazaki C, Ashihara E, Ohkawa K, Oku N, Inaba T, Murakami S, Fujita N, Nakagawa M
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
Acta Haematol. 1992;88(2-3):129-35. doi: 10.1159/000204668.
The effects of interleukin-3 (IL-3) and interleukin-6 (IL-6) on nonadherent mononuclear cells (NMC) from the peripheral blood of 28 patients with multiple myeloma (MM), 3 patients with monoclonal gammopathy of undetermined significance (MGUS), and 3 normal controls were investigated. In 15 of 27 evaluable patients with MM, monoclonal-cytoplasmic-immunoglobulin (cIg)-positive plasma cells appeared from the T-cell-depleted NMC after 10 days of culture in the presence of IL-3 and IL-6. These changes were not observed in the T cell fraction of myeloma blood or in the T-cell-depleted NMC obtained from cases of MGUS or from normal controls. The percentage of cIg-positive plasmacytoid cells after 10 days of culture was significantly higher in the presence of both IL-3 and IL-6 than with each interleukin alone or the control medium. Furthermore, these changes were often observed in untreated patients. These findings suggest that myeloma precursor cells exist in the peripheral blood of MM patients, especially at diagnosis, and differentiate into cIg-positive cells in the presence of IL-3 and IL-6. This assay may be useful in discriminating the early stage of myeloma from MGUS.
研究了白细胞介素-3（IL-3）和白细胞介素-6（IL-6）对28例多发性骨髓瘤（MM）患者、3例意义未明的单克隆丙种球蛋白病（MGUS）患者及3名正常对照者外周血中非黏附单核细胞（NMC）的影响。在27例可评估的MM患者中，有15例患者的T细胞去除的NMC在添加IL-3和IL-6的情况下培养10天后出现了单克隆细胞质免疫球蛋白（cIg）阳性浆细胞。在骨髓瘤患者血液的T细胞组分中，以及从MGUS患者或正常对照者获得的T细胞去除的NMC中均未观察到这些变化。培养10天后，同时存在IL-3和IL-6时，cIg阳性浆细胞样细胞的百分比显著高于单独使用每种白细胞介素或对照培养基时。此外，这些变化在未经治疗的患者中也经常观察到。这些发现表明，MM患者外周血中存在骨髓瘤前体细胞，尤其是在诊断时，并且在IL-3和IL-6存在的情况下可分化为cIg阳性细胞。该检测方法可能有助于鉴别骨髓瘤与MGUS的早期阶段。
