Tatsumi T, Shimazaki C, Goto H, Araki S, Sudo Y, Yamagata N, Ashihara E, Inaba T, Fujita N, Nakagawa M
Second Department of Medicine, Kyoto Prefectural University of Medicine.
Jpn J Cancer Res. 1996 Aug;87(8):837-42. doi: 10.1111/j.1349-7006.1996.tb02108.x.
We investigated the expression of adhesion molecules including LFA-1 alpha (CD11a), Mac-1 (CD11b), LFA-1 beta (CD18), VLA-beta 1 (CD29), H-CAM (CD44), VLA-4 (CD49d), VLA-5 (CD49e), ICAM-1 (CD54), N-CAM (CD56), LFA-3 (CD58), VNR-beta (CD61), and LECAM-1 (CD62L) on fresh myeloma cells and human myeloma cell lines. By two-color flow cytometric analysis with anti-CD38 antibody, we demonstrated that myeloma cells were located in the strongly CD38-positive (CD38++) fractions. Fresh myeloma cells were obtained from 28 patients with multiple myeloma (MM) and 3 patients with plasma cell leukemia (PCL). All myeloma cells expressed VLA-4 on their surface. Most of the myeloma cells also expressed VLA-5, ICAM-1, and LFA-3, H-CAM was strongly expressed in 3 cases of PCL and 2 cases of aggressive myeloma, and moderately expressed in other MMs. N-CAM was expressed in 68% of MMs, but none of the 3 PCLs. LFA-1 was expressed in two cases of aggressive myeloma, but not expressed in other non-aggressive myelomas. Most of the myeloma cells did not express Mac-1, VNR-beta, or LECAM-1. These results suggest that VLA-4, VLA-5, ICAM-1, LFA-3, and H-CAM are involved in cellular interaction and migration in MM, and that the expression of N-CAM and LFA-1 varies with disease activity in MM.
我们研究了包括淋巴细胞功能相关抗原-1α（CD11a）、巨噬细胞-1（CD11b）、淋巴细胞功能相关抗原-1β（CD18）、极迟抗原-β1（CD29）、造血细胞粘附分子（CD44）、极迟抗原-4（CD49d）、极迟抗原-5（CD49e）、细胞间粘附分子-1（CD54）、神经细胞粘附分子（CD56）、淋巴细胞功能相关抗原-3（CD58）、玻璃体结合蛋白受体-β（CD61）和淋巴细胞内皮细胞粘附分子-1（CD62L）在内的粘附分子在新鲜骨髓瘤细胞和人骨髓瘤细胞系上的表达。通过用抗CD38抗体进行双色流式细胞术分析，我们证明骨髓瘤细胞位于强CD38阳性（CD38++）部分。新鲜骨髓瘤细胞取自28例多发性骨髓瘤（MM）患者和3例浆细胞白血病（PCL）患者。所有骨髓瘤细胞表面均表达极迟抗原-4。大多数骨髓瘤细胞还表达极迟抗原-5、细胞间粘附分子-1和淋巴细胞功能相关抗原-3，造血细胞粘附分子在3例PCL和2例侵袭性骨髓瘤中强表达，在其他MM中中度表达。68%的MM表达神经细胞粘附分子，但3例PCL均未表达。淋巴细胞功能相关抗原-1在2例侵袭性骨髓瘤中表达，但在其他非侵袭性骨髓瘤中未表达。大多数骨髓瘤细胞不表达巨噬细胞-1、玻璃体结合蛋白受体-β或淋巴细胞内皮细胞粘附分子-1。这些结果表明，极迟抗原-4、极迟抗原-5、细胞间粘附分子-1、淋巴细胞功能相关抗原-3和造血细胞粘附分子参与了MM中的细胞相互作用和迁移，并且神经细胞粘附分子和淋巴细胞功能相关抗原-1的表达随MM中的疾病活动而变化。
