Parfënova E V, Pisareva L N
Tsitologiia. 1975 May;27(5):539-44.
The treatment of membrane suspension from the guinea pig kidney cortex possessing Na+, + K+ -ATPase activity with 0.20% sodium deoxycholate (DOX : protein equals 12 : 1) leads to lowering of their enzymatic activity. DOX-treated preparation can be divided into two fractions by centrigugation (1 hr - 22 000 g). The pellet has 1.5-fold higher enzymatic activity than the initial preparation and cannot be activated by small amounts of histones. The supernatant has 2-fold lower enzymatic activity than the initial preparation but in presence of small amounts of histones this activity rises to the level of the initial preparation activity. The action of DOX treatment on the membrane structure of Na+ + K+ -ATPase preparations is discussed.
用0.20%的脱氧胆酸钠（DOX与蛋白质的比例为12:1）处理具有Na⁺、K⁺ -ATP酶活性的豚鼠肾皮质膜悬浮液，会导致其酶活性降低。经DOX处理的制剂通过离心（1小时 - 22000克）可分为两部分。沉淀的酶活性比初始制剂高1.5倍，且不能被少量组蛋白激活。上清液的酶活性比初始制剂低2倍，但在存在少量组蛋白的情况下，该活性会升至初始制剂活性水平。文中讨论了DOX处理对Na⁺、K⁺ -ATP酶制剂膜结构的作用。
