Vasilenko T F, Pisareva L N, Skvortsevich E G
Tsitologiia. 1982 Apr;24(4):492-6.
The effect of pH in the incubation medium on ATPase activity and the direct 18O-exchange, catalyzed by initial and treated by surface active agents membrane preparations of Na, K-ATPase has been studied. The pH dependence of the ratio between the direct 18O exchange and Na,K-ATPase activity was detected; this ratio being equal to 4,5 +/- +/- 0.6, 1.5 +/- 0.1 and 0.4 +/- 0.1 at the pH values of 6.1, 7.5 and 9.0, respectively. The pretreatment of preparations by DOC or histone H2a, used in both activatory and inhibitory-amounts, abolished the effect of acid pH and made the ratio practically the same at 6.1 and 7.5 pH values. Both agents did not affect the action of the alcaline pH value equal to 9.0. Triton X-100 was not able to change the pH dependence of the ratio.
研究了孵育介质的pH值对Na，K - ATP酶活性以及由初始和经表面活性剂处理的膜制剂催化的直接18O交换的影响。检测了直接18O交换与Na，K - ATP酶活性之间比率的pH依赖性；在pH值为6.1、7.5和9.0时，该比率分别等于4.5±0.6、1.5±0.1和0.4±0.1。用激活量和抑制量的去氧胆酸钠（DOC）或组蛋白H2a对制剂进行预处理，消除了酸性pH的影响，并使在pH值6.1和7.5时的比率几乎相同。这两种试剂均不影响等于9.0的碱性pH值的作用。Triton X - 100不能改变该比率的pH依赖性。
