Charnock C, Bergan T
Department of Microbiology, University of Oslo, Norway.
Eur J Clin Microbiol Infect Dis. 1992 Sep;11(9):810-6. doi: 10.1007/BF01960880.
Multilocus enzyme electrophoresis was used to characterize 27 Pseudomonas aeruginosa serogroup reference strains used in the major O-antigen schemes according to which Pseudomonas aeruginosa has been typed. Sixteen enzyme loci were assayed, ten of which showed electrophoretic variation. Genetic diversity was expressed for each enzyme locus, and as the mean allelic diversity of loci. Ten electrophoretic types were identified among the strains. The genetic distance between pairs of electrophoretic types was expressed as the proportion of loci at which similar alleles occurred. More than 80% similarity was observed between any pair of electrophoretic types, reflecting the homogeneity of multilocus genotypes within this species. Similarity between electrophoretic types was represented in the form of a dendrogram and by multi-dimensional scaling. Three distinct clusters of electrophoretic types were revealed; within each the serogroups appeared to be randomly distributed.
多位点酶电泳用于对27株铜绿假单胞菌血清群参考菌株进行特征分析，这些菌株用于主要的O抗原分型方案，根据该方案对铜绿假单胞菌进行了分型。检测了16个酶位点，其中10个显示出电泳变异。对每个酶位点表达了遗传多样性，并作为位点的平均等位基因多样性。在这些菌株中鉴定出了10种电泳类型。成对电泳类型之间的遗传距离表示为出现相似等位基因的位点比例。在任何一对电泳类型之间观察到超过80%的相似性，反映了该物种内多位点基因型的同质性。电泳类型之间的相似性以树状图和多维标度的形式表示。揭示了三个不同的电泳类型簇；在每个簇内，血清群似乎是随机分布的。
