Mizumoto Hiroyuki, Mizumoto Keiko, Shatos Marie A, Klassen Henry, Young Michael J
The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, 20 Staniford Street, Boston, MA 02114, USA.
Vision Res. 2003 Jul;43(16):1699-708. doi: 10.1016/s0042-6989(03)00235-9.
Neural progenitor cells isolated from the brains of neonatal GFP transgenic mice were grafted to the retina of RCS rats and rds and B6 mice. Expression of GFP and differentiation markers was evaluated at 1-4 weeks post-transplantation. Grafted cells maintained transgene expression throughout the 4-week period. At 1 week there was widespread migration of GFP+cells within the host retina and at 2 weeks evidence of neuronal differentiation (as shown by both marker expression and cell morphology), although integration at 4 weeks was limited to syngeneic recipients. Because brain-derived neural progenitor cells exhibit both neuronal and astrocytic differentiation in diseased and normal host retina, these cells provide a useful tool for studies of retinal regeneration.
从新生绿色荧光蛋白（GFP）转基因小鼠大脑中分离出的神经祖细胞被移植到视网膜色素变性（RCS）大鼠以及视网膜退化慢（rds）小鼠和B6小鼠的视网膜中。在移植后1至4周评估GFP和分化标志物的表达情况。移植细胞在整个4周期间都维持转基因表达。在第1周时，GFP+细胞在宿主视网膜内广泛迁移，到第2周时有神经元分化的证据（通过标志物表达和细胞形态均显示），尽管在第4周时整合仅局限于同基因受体。由于源自大脑的神经祖细胞在患病和正常宿主视网膜中均表现出神经元和星形胶质细胞分化，这些细胞为视网膜再生研究提供了一个有用的工具。
