Martins M C L, Fonseca C, Barbosa M A, Ratner B D
INEB-Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal.
Biomaterials. 2003 Sep;24(21):3697-706. doi: 10.1016/s0142-9612(03)00244-8.
Chronopotentiometry was used to study the adsorption of human serum albumin (HSA) to self-assembled monolayers with the following terminal functional groups: CH(3), COOH and OH. Surfaces were characterized by X-ray photoelectron spectroscopy, water contact angle measurements and cyclic voltammetry. HSA coverage of the different SAMs was investigated by chronopotentiometry and the total amount of adsorbed protein was determined using radiolabelled albumin. Both techniques have demonstrated that HSA adsorption to the different SAM-modified electrodes increases in the following order: OH<COOH<CH(3)-terminated SAMs. A good correlation between coverage and total amount of HSA adsorbed was observed for long adsorption times (900s).
采用计时电位分析法研究了人血清白蛋白（HSA）在具有以下末端官能团的自组装单分子层上的吸附情况：CH(3)、COOH和OH。通过X射线光电子能谱、水接触角测量和循环伏安法对表面进行了表征。通过计时电位分析法研究了不同自组装单分子层（SAMs）上HSA的覆盖情况，并使用放射性标记的白蛋白测定了吸附蛋白质的总量。两种技术均表明，HSA在不同SAM修饰电极上的吸附按以下顺序增加：OH<COOH<CH(3)封端的SAMs。在较长吸附时间（900秒）下，观察到HSA覆盖度与吸附总量之间具有良好的相关性。
