Matsuda H, Matsumoto M, Kitahara H, Haraguchi S, Kanai K
Second Department of Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):654-61. doi: 10.1016/0006-291x(92)92251-r.
A rat hepatoma cell line, FF101, established in serum-free, protein-free medium, synthesizes a growth factor(FF-GF). FF-GF was purified by gel filtration chromatography, cation-exchange chromatography and isoelectric focusing. Purified FF-GF was revealed as a single band on SDS-gel electrophoresis and its molecular weight was estimated to be 70 KDa. FF-GF stimulated DNA synthesis of various cells from different origins. The growth-promoting activities of FF-GF were abolished by treating with protease, dithiothreitol, acid and heating, whereas its activity was not inhibited by antibodies against acidic and basic fibroblast growth factors. These results indicate that FF-GF is a novel growth factor.
一种在无血清、无蛋白培养基中建立的大鼠肝癌细胞系FF101，能合成一种生长因子（FF-GF）。FF-GF通过凝胶过滤色谱、阳离子交换色谱和等电聚焦进行纯化。纯化后的FF-GF在SDS凝胶电泳上显示为单一条带，其分子量估计为70 kDa。FF-GF刺激来自不同来源的各种细胞的DNA合成。用蛋白酶、二硫苏糖醇、酸处理和加热会消除FF-GF的促生长活性，而其活性不受抗酸性和碱性成纤维细胞生长因子抗体的抑制。这些结果表明FF-GF是一种新型生长因子。
