Checiówna D, Klein A
Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Folia Histochem Cytobiol. 1996;34(3-4):135-40.
Transformation of rat cells by Rous sarcoma virus(es) induced the release of growth factors into serum-free conditioned media. An PR-RSV-transformed rat cell line, XC, produced and released polypeptide factors which promote anchorage-dependent and anchorage-independent growth of XC cells. One of the autocrine factors of XC cells was purified to homogeneity by four-step procedure: ultrafiltration, ion-exchange chromatography on MonoS, reverse-phase chromatography on Spherisorb ODS2 and gel filtration on Superose 12. The factor gave a single band on SDS-electrophoresis on polyacrylamide gel and was assumed to have a molecular weight of 16 kDa. The factor is a potent mitogen for XC cells; half-maximal stimulation of DNA synthesis was achieved at a concentration of 0.8 ng/ml. The peptide is probably one of the family of EGF-like heparin-binding growth factors.
劳氏肉瘤病毒诱导大鼠细胞发生转化，致使生长因子释放到无血清条件培养基中。一种PR-RSV转化的大鼠细胞系XC，产生并释放出能促进XC细胞贴壁依赖性生长和非贴壁依赖性生长的多肽因子。通过超滤、MonoS离子交换色谱、Spherisorb ODS2反相色谱和Superose 12凝胶过滤这四个步骤，将XC细胞的一种自分泌因子纯化至同质。该因子在聚丙烯酰胺凝胶SDS电泳上呈现单一条带，推测其分子量为16 kDa。该因子是XC细胞的一种强效促有丝分裂原；在浓度为0.8 ng/ml时可实现DNA合成的半数最大刺激。该肽可能是表皮生长因子样肝素结合生长因子家族的一员。
