2'-脱氧助间型霉素对博来霉素诱导的DNA链断裂修复的抑制作用及其对L5178Y淋巴母细胞抗肿瘤活性的影响。
Inhibition of repair of bleomycin-induced DNA strand breaks by 2'-deoxycoformycin and its effect on antitumor activity in L5178Y lymphoblasts.
作者信息
Begleiter A, Peniuk H, Israels L G, Johnston J B
机构信息
Manitoba Institute of Cell Biology, Manitoba Cancer Treatment and Research Foundation, Winnipeg, Canada.
出版信息
Biochem Pharmacol. 1992 Dec 1;44(11):2229-33. doi: 10.1016/0006-2952(92)90351-i.
We have observed previously that treatment of plateau-phase L5178Y murine lymphoblasts in vitro with 2'-deoxycoformycin plus deoxyadenosine (dCF/dAdo) can inhibit the repair of X-irradiation-induced DNA single-strand breaks (SSB) in these cells and that this effect is associated with synergistic cell kill. In this study we examined the effect of a combination treatment of plateau-phase L5178Y cells with bleomycin (BLM) plus dCF/dAdo. Incubation of BLM-treated cells with dCF/dAdo resulted in significant inhibition of the repair of BLM-induced DNA SSB. However, an additive, but not a synergistic, increase in cell kill was observed when cells were treated with a combination of BLM plus dCF/dAdo.
我们之前观察到，用2'-脱氧助间型霉素加脱氧腺苷（dCF/dAdo）体外处理处于平台期的L5178Y小鼠淋巴细胞，可抑制这些细胞中X射线诱导的DNA单链断裂（SSB）的修复，且这种效应与协同性细胞杀伤有关。在本研究中，我们检测了博来霉素（BLM）加dCF/dAdo联合处理处于平台期的L5178Y细胞的效应。用dCF/dAdo孵育经BLM处理的细胞，可显著抑制BLM诱导的DNA SSB的修复。然而，当细胞用BLM加dCF/dAdo联合处理时，观察到细胞杀伤作用呈相加而非协同增强。
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