Müller W E, Zahn R K, Maidhof A, Schröder H C, Bachmann M, Umezawa H
J Antibiot (Tokyo). 1984 Mar;37(3):239-43. doi: 10.7164/antibiotics.37.239.
Studying the treatment of NMRI mice with ip injections of bleomycin (BLM) for 5 days we found an approximate LD50 of 35 mg/kg; the toxicity of peplomycin (PEP) was slightly higher (LD50: approximately 25 mg/kg). The effect of the two drugs on growth of L5178y mouse lymphoma cells in NMRI mice was examined. BLM alone caused at a concentration of 2.5 mg/kg an almost complete inhibition of tumor cell growth; the same effect was determined with 1 mg PEP/kg. At these concentrations the drugs caused an increase of the survival time of 110% (BLM) or 104% (PEP). Given in combination, one-sixth of the optimal doses yielded an 100% increase of the median survival time. These results indicate a significant synergistic activity of the PEP-BLM combination on L5178y cell growth in vivo (FIC index: 0.34).
通过对NMRI小鼠进行5天的博来霉素（BLM）腹腔注射治疗研究，我们发现其半数致死量（LD50）约为35mg/kg；培普利欧霉素（PEP）的毒性略高（LD50：约25mg/kg）。研究了这两种药物对NMRI小鼠体内L5178y小鼠淋巴瘤细胞生长的影响。单独使用BLM时，浓度为2.5mg/kg几乎能完全抑制肿瘤细胞生长；使用1mg PEP/kg时也能产生相同效果。在这些浓度下，药物使存活时间延长了110%（BLM）或104%（PEP）。联合使用时，最佳剂量的六分之一可使中位存活时间提高100%。这些结果表明，PEP - BLM组合对体内L5178y细胞生长具有显著的协同活性（联合抑菌系数：0.34）。
