Oremek G M, Gerstmeier F, Sauer-Eppel H, Sapoutzis N, Wechsel H W
Klinikum der Johann-Wolfgang Goethe-Universität Frankfurt, Zentrallabor-Zentrum Innere Medizin, Theodor-Stern-Kai 7, D-60590 Frankfurt/Main, Germany.
Anticancer Res. 2003 Mar-Apr;23(2A):1127-30.
Tumor M2-PK is an isoform of the glycolytic enzyme pyruvate kinase. This isoform exists in an active tetrameric and less active dimeric form. The dimeric form is expressed by various tumor cells and can be measured in blood by a specific ELISA.
We included 500 healthy persons, 20 patients with an acute rheumatic disease (high CRP) and 30 patients with a nephropathy and proteinuria with more than 20 mg/dl. We measured Tumor M2-PK in each of these groups in different specimens of EDTA-plasma, serum, heparin-, citrate-, fluoride- and oxalate-plasma.
We found different concentrations of Tumor M2-PK in healthy persons depending on the kind of specimen. The normal range of Tumor M2-PK concentration was higher in the serum than in the plasma, with 35 U/l compared to 15 U/l. In haemolytic material we found concentrations up to 80 U/l in healthy persons. Lipaemic or icteric serum material showed a higher concentration of Tumor M2-PK as well. In patients with a triglyceride concentration higher than 300 mg/dl, the normal range for Tumor M2-PK was measured between 30-50 U/l. Similar results were found in patients with icteric serum. Patients with an active rheumatic disease and elevated CRP concentration showed a Tumor M2-PK concentration between 40-60 U/l. Patients with a nephropathy and proteinuria over 20 mg/dl had an elevated Tumor M2-PK concentration from 25-60 U/l.
We conclude that different materials seem to be suitable for the measurement of Tumor M2-PK concentration as long as attention is paid to different normal ranges. Haemolytic material should not be used as it offers false-positive results. Some diseases such as rheumatic disease, hyperlipidaemia and nephropathy have an influence on Tumor M2-PK concentration that should not be neglected.
肿瘤M2-PK是糖酵解酶丙酮酸激酶的一种同工型。这种同工型以活性四聚体和活性较低的二聚体形式存在。二聚体形式由各种肿瘤细胞表达，可通过特异性酶联免疫吸附测定法在血液中检测到。
我们纳入了500名健康人、20名患有急性风湿性疾病（高CRP）的患者以及30名患有肾病且蛋白尿超过20mg/dl的患者。我们在这些组中的每一组中，对不同标本（EDTA血浆、血清、肝素血浆、枸橼酸盐血浆、氟化物血浆和草酸盐血浆）进行肿瘤M2-PK检测。
我们发现健康人中肿瘤M2-PK的浓度因标本类型而异。肿瘤M2-PK浓度的正常范围血清高于血浆，血清为35U/l，血浆为15U/l。在健康人的溶血材料中，我们发现浓度高达80U/l。脂血或黄疸血清材料中肿瘤M2-PK的浓度也较高。甘油三酯浓度高于300mg/dl的患者，肿瘤M2-PK的正常范围在30 - 50U/l之间。黄疸血清患者也有类似结果。患有活动性风湿性疾病且CRP浓度升高的患者，肿瘤M2-PK浓度在40 - 60U/l之间。患有肾病且蛋白尿超过20mg/dl的患者，肿瘤M2-PK浓度升高，在25 - 60U/l之间。
我们得出结论，只要注意不同的正常范围，不同材料似乎都适合用于检测肿瘤M2-PK浓度。不应使用溶血材料，因为它会给出假阳性结果。一些疾病，如风湿性疾病、高脂血症和肾病，对肿瘤M2-PK浓度有影响，不容忽视。
